Reaggregate Thymus Cultures

Reaggregate thymus cultures.

Stromal cells within lymphoid tissues are organized into three-dimensional structures that provide a scaffold that is thought to control the migration and development of haemopoeitic cells. Importantly, the maintenance of this three-dimensional organization appears to be critical for normal stromal cell function, with two-dimensional monolayer cultures often being shown to be capable of support...

Coxsackievirus B4 infection of murine foetal thymus organ cultures.

The infection of foetal thymus with coxsackievirus B4 (CV-B4) E2 has been studied ex vivo by using CD-1 mice on foetal day 14, as a ready source of organs for experimentation to investigate the hypothesis of the role of thymic viral infections in the pathogenesis of type 1 diabetes. The replication of CV-B4 E2 in murine foetal thymus organ cultures has been demonstrated by evaluating the levels...

Cultured heart cell reaggregate model for studying cardiac toxicology.

This review represents a summary of the technique of using cultured heart cells as a model system for studying the physiology, pharmacology, biochemistry and toxicology of myocardial cells. The general techniques and types of culture preparations commonly used are given and some of the advantages and disadvantages of working with cultured heart cells are summarized.

The transformed cell in thymus-PHA cultures: blood or tissue origin?

Bone marrow-derived cells fail to induce positive selection in thymus reaggregation cultures

The requirements for inducing positive selection of T cells were examined in thymus reaggregation cultures, a system in which dispersed populations of immature CD4+8+ cells and purified thymic epithelial cells (TEC) are reaggregated in tissue culture. Studies with TEC from mice selectively lacking major histocompatibility complex (MHC) class I (I-II+), class II (I+II-), or both class I and II (...